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Chapter 1
Immunoaffinity Capture of Cells from Whole Blood
| 1.1 |
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Introduction |
| 1.2 |
Experimental Design |
| 1.3 |
Materials |
| 1.4 |
Methods |
| 1.4.1 |
Device Fabrication |
| 1.4.2 |
Surface modification |
| 1.4.3 |
Cell capture |
| 1.4.4 |
Post-capture processing |
| 1.5 |
Data Acquisition, anticipated results, and interpretation |
| 1.6 |
Discussion and commentary |
| 1.7 |
Troubleshooting table |
| 1.8 |
Application notes |
| 1.9 |
Summary Points |
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Chapter 2
Dynamic Gene Expression Analysis in a Living Cell Array
| 2.1 |
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Introduction |
| 2.2 |
Materials |
| 2.3 |
Methods |
| 2.3.1 |
GFP Reporter Cell Line Construction |
| 2.3.2 |
Microfluidic Cell Array Fabrication |
| 2.3.3 |
Microfluidic Array Pretreatment and Seeding |
| 2.3.4 |
Stimulation and Reporter Imaging |
| 2.4 |
Data Acquisition, Anticipated Results And Interpretation |
| 2.5 |
Discussion |
| 2.6 |
Troubleshooting Table |
| 2.7 |
Application Notes |
| 2.8 |
Summary Points |
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