Methods in Bioengineering: 3D Tissue Engineering
Francois Berthiaume, Rutgers University and Jeffrey Morgan, Brown University, Editors
ISBN: 978-1-59693-458-0
Pages: 280

Tissue engineering is an emerging field that involves the combination of materials, cells, and other signals or growth factors to generate new tissue that can be used to repair or replace damaged tissues due to injury or disease. This groundbreaking volume presents the latest methods and protocols for systematically building tissues in 3D configuration outside the body, as well as providing techniques that modulate repair and regeneration processes that occur “in situ” (in their natural or original place).

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Methods in Bioengineering: Alternative Technologies to Animal Testing
Tim Maguire and Eric Novik, Editors
ISBN: 978-1-60807-011-4 
Pages: 294

Providing alternatives to animal testing is one of the hottest topics in biomedical research, and this groundbreaking volume addresses this critical issue head on. This unique book presents techniques and methods at the forefront of scientific research that have the potential to replace certain whole animal tests. Moreover, this book provides a platform where other widely accepted techniques and scientific advancements can be collated into a concise set of methods that can be implemented within both academic and industrial communities.

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Methods in Bioengineering: Biomicrofabrication and Biomicrofluidics
Jeffrey D. Zahn, Rutgers University, Editor
ISBN: 978-1-59693-400-9
Pages: 380

Microfluidics promises to transform the field of medicine by dramatically changing the ways in which diagnostics and clinical research are conducted in hospitals and laboratories. This unique volume presents leading-edge microfluidics methods used to handle, manipulate, and analyze cells, particles, and biological components (e.g., proteins and DNA) for microdiagnostics. The authors offer a clear and detailed guidance on:

• Microfabrication techniques utilized to create microfluidic devices and on-chip flow control and mixing Microsystems.
• Protein and DNA handling devices for electrophoretic and isoelectric separations in microchromatography columns.
• Microfluidic manipulations of droplets via electrowetting and particles via dielectrophoresis for separations and chemical reactions.
• Integrated optical characterization of microfluidic devices.

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Methods in Bioengineering: Nanoscale Bioengineering and Nanomedicine
Kaushal Rege, Arizona State University and Igor L. Medintz, U.S. Naval Research Laboratory
ISBN: 978-1-59693-410-8 
Pages: 332 pp.

Filling a critical gap in the current literature, this new resource presents practical, step-by-step methods to help you synthesize, characterize, biofunctionalize and apply the nanomaterial that is most suitable for handling a given nanoscale bioengineering problem. Written and presented by leading scientists and engineers in their respective fields, the authors offer a clear and detailed understanding of how to carry out:

• Nanoparticle functionalization with biomolecules, including enzymes
• Nanoparticle analysis and characterization
• In vitro evaluation of nanoparticles using different cell lines
• In vitro evaluation of nanoparticles as therapeutics and imaging agents

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Methods in Bioengineering: Systems Analysis of Biological Networks
Arul Jayaraman and Juergen Hahn, Texas A&M University
ISBN: 978-1-59693-406-1
Pages: Approx. 330 pp.

This cutting-edge volume provides a detailed look at the two main aspects of systems biology: the design of sophisticated experimental methods and the development of complex models to analyze the data. Focusing on methods that are being used to solve current problems in biomedical science and engineering, this comprehensive, richly illustrated resource shows you how to:

• Design of state-of-the art methods for analyzing biological systems
• Implement experimental approaches for investigating cellular behavior in health and disease
• Use algorithms and modeling techniques for quantitatively describing biomedical problems
• Integrate experimental and computational approaches for a more complete view of biological systems

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Methods in Bioengineering: Microdevices in Biology and Medicine
Yaakov Nahmias, Massachusetts General Hospital/Harvard Medical School and Sangeeta Bhatia, MIT
ISBN: 978-1-59693-404-7
Pages: Approx. 365 pp.

At the dawn of the 21st century, microtechnology is changing the study of biology and the practice of medicine. Offering an unparalleled, practical look into the field, this unique volume presents the science behind microscale device design and the engineering of its fabrication. Supported with dozens of full-color illustrations, the book presents clear, step-by-step methods for:

• Cell capture from whole blood.
• High-throughput study of transcriptional dynamics in living cells.
• Temporal control of cell-cell interaction.
• Nanoscale measurements of cellular forces.
• Immobilizing living c. elegans.
• Optical and electrical on-chip cell sorting.
• Human-on-chip modeling of drug metabolism.

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Methods in Bioengineering: Stem Cell Bioengineering
Biju Parekkadan and Martin Yarmush, Massachusetts General Hospital/Harvard Medical School, Editors
ISBN: 978-1-59693-402-3
Pages: Approx. 365 pp.

In stem cell research there are several key methods that, once mastered, can be extremely powerful. These methods enable one to rigorously test hypotheses, compare results to "gold standards" and may spur investigators to improve existing protocols. This book describes numerous methods to derive, manipulate, target and prepare stem cells for clinical use. The methods described here help researchers:

• Derive and test human embryonic stem cells.
• Analyze bone marrow stem cell function in vitro and in vivo.
• Image a stem cell transplant.
• Cryopreserve stem cells.
• Differentiate stem cells using microscale techniques.

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