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Methods in Bioengineering: Stem Cell Bioengineering
Biju Parekkadan and Martin Yarmush, Mass General Hospital/Harvard Medical School, Editors
ISBN 978-1-59693-402-3
Copyright 2009
Pages: 260
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  • Description
  • Editors
  • Contents

In stem cell research there are several key methods that, once mastered, can be extremely powerful. These methods enable you to rigorously test hypotheses, compare results to “gold standards,” and may even spur improvements to existing protocols. This book describes numerous methods to derive, manipulate, target, and prepare stem cells for clinical use. The methods described here help you:

  • Derive and test human embryonic stem cells.
  • Analyze bone marrow stem cell function in vitro and in vivo.
  • Image a stem cell transplant.
  • Cryopreserve stem cells.
  • Differentiate stem cells using microscale techniques

    • Related Titles
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    Methods in Bioengineering: Biomicrofabrication and Biomicrofluidics by Jeffrey D. Zahn, Editor
    Methods in Bioengineering: Microdevices in Biology and Medicine by Yaakov Nahmias and Sangeeta Bhatia
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    Biju Parekkadan is an instructor in surgery and bioengineering at Massachusetts General Hospital. His research focus is in the area of stem cell bioengineering. He holds a B.S. in biomedical engineering from Rutgers University and a Ph.D. in chemical and medical engineering from the Harvard-MIT Division of Health Sciences and Technology.

    Martin Yarmush is the Helen Andrus Benedict Professor of Surgery and Bioengineering at the Harvard Medical School and Director of the Center for Engineering in Medicine at Massachusetts General Hospital. Dr. Yarmush is an internationally recognized engineer and biophysical chemist. He is known as one of the leading investigators in the area of molecular and cellular bionengineering through seminal contributions to the fields of hepatic and skin tissue engineering, applied immunology, metabolic engineering, and geonomics and proteomics.

    Overview. Derivation of Human Embryonic Stem Cell Lines. Clinical Scale Culture Methods for Stem Cells. Microscal Methods to Probe Stem Cell Differentiation and Function. Isolation, Analysis and Ex Vivo Expansion of Adult Stem Cells. Boosting Endogenous Stem Populations in the Adult. Graft Preparation and Administration. Imaging Stem Cell Transplants.

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