This groundbreaking work puts you at the cutting edge of diagnostic and therapeutic bioengineering, and brings you up to speed with the extraordinary range of cell-based biomedical applications now in development. Written by pioneers in the field, it explores the top-down and bottom-up technologies that can be used to control various aspects of the stem cell microenvironment. The book shows how these microfabrication and material synthesis techniques are paving the way to dramatic clinical breakthroughs in areas ranging from in vitro fertilization to artificial skin and organ tissue generation.
Supported with 140 illustrations, the volume exhaustively covers the micro- and nano-system technologies involved in developing cell-based bioengineering applications. You get full details on efforts to engineer the soluble and insoluble cell microenvironments, including the latest advances in microfluidic devices, surface patterning, 3D scaffolds, and techniques for engineering cellular mechanical properties and topography. The book systematically reviews emerging biomedical applications, including the latest in embryonic cell development, microfabrication of artificial neural tissue, and microvascular and cardiac tissue engineering. What’s more, this comprehensive resource covers hydrogels for cartilage tissue, bone tissue engineering, kidney replacement therapies, and pulmonary applications. Moreover, the book presents the latest advances in hepatic tissue engineering, immune cell analysis, and the development of engineered skin substitutes.
Introduction to Engineering the Cellular Microenvironment.
Part I: Technologies
Engineering the Soluble Microenvironment of Cells—Gradient-Generating Microfluidic Devices for Cell Biology Research
Engineering the Insoluble Biochemical Microenvironment of Cells—Surface Patterns: Surface Patterning for Controlling Cell-Substrate Interactions. Patterned Co-cultures for Controlling Cell-Cell Interactions. 3D Scaffolds: Micro- and Nano-fabricated Scaffolds for 3D Tissue Recapitulation. Engineered Hydrogels for 3D Cell Culture. 3D Cell Printing Technologies for Tissue Engineering.
Engineering the Biophysical Cues of the Cellular Microenvironment—Mechanical Properties: Using Microfabrication to Engineer Cellular and Multicellular Architecture. Engineering Substrate Mechanics to Regulate Cell Response. Topography: Generation of Surface Nanotopography for Controlling Cell-Substrate Interactions.
Part II: Applications
Embryonic Cell Development/Fertilization—Microfluidics for Assisted Reproductive Technologies. Microscale Technologies for Engineering of Embryonic Stem Cell Environments.
Neural—Neuroscience on a Chip: Microfabrication for In-vitro Neurobiology. Self-assembly of Nanomaterials for Engineering Cell Microenvironment.
Vascular—Microvascular Engineering: Design, Modeling, and Microfabrication. Nanotechnology for Inducing Angiogenesis.
Muscle—Micropatterning Approaches for Cardiac Biology. Microreactors. Microreactors for Cardiac Tissue Engineering.
Cartilage—Nanoengineered Hydrogels for Cartilage Tissue Engineering.
Bone—Microscale Approaches for Bone Tissue Engineering. Nanoengineering for Bone Tissue Engineering. Bioinspired Engineered Nanocomposites for Bone Tissue Engineering.
Kidney—Technological Approaches to Renal Replacement Therapies.
Pulmonary—Engineering Pulmonary Epithelia and Their Mechanical Microenvironments.
Immune System—Microfabricated Systems for Analyzing Immune Cell Functions.
Liver—Microscale Hepatic Tissue Engineering
Skin—Nano- and Microtechnologies for the Development of Engineered Skin Substitutes.
Summary and Outlook.
Ali Khademhosseini is an assistant professor of medicine and health sciences and technology at Brigham and Women’s Hospital, Harvard Medical School. He is the co-author of several papers in the field, was recognized as one of the top young innovators (TR35) by Technology Review magazine and was a keynote speaker at the 2006
International Digital Fabrication Conference. He received his Ph.D. in Bioengineering from the Massachusetts Institute of Technology.
Jeffrey Borenstein is a distinguished member of the technical staff and director of the Biomedical Engineering Center at Draper Laboratory, Cambridge, MA. Dr. Borenstein is also the associate director of the Center for Integration of Medicine and Innovative Technology, where he is co-principle investigator in the CIMIT Tissue Engineering Project. He earned his Ph.D. in physics at the University at Albany.
Mehmet Toner is a professor of surgery at Massachusetts General Hospital, Harvard Medical School and president of the Society for Cryobiology. He earned his Ph.D. in medical engineering at the Massachusetts Institute of Technology.
Shuichi Takayama is an associate professor of biomedical engineering and macromolecular science and engineering at the University of Michigan. He earned his Ph.D. in chemistry from The Scripps Research Institute.
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